A Simple Solution for Oligonucleotide Design

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Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SG0006Publication date: 2002
PMID: 12208112GI: 2337967 Taxonomy ID: 35252
Virus Name: Alcelaphine herpesvirus 1Product size (PCR): 386 bp
MgCl2: 1.5 mMdNTPs: mMType: PCR
Temp (PCR cycle):95C 5min>(94C 1min>45C 1min>72C 1min)*24>94C 1min>45C 1min>72C 5min
Buffer:
Polymerase: 2U Taq DNA polyCycler:
Note: 2microG of DNA was also included in the reaction mix. After the first full amplification 2microL of the first amplification was added to a new amplification mixture and the process was repeated.
Forward(POL-1) primer
Oligo type: Forward PrimerDegeneracy: 8
Target: 21267-21286Tm: 80.4C at salt 1000.0 mMLength:
Sequence: GGC(C/T)CA(C/T)AA(C/T)CTATGCTACTCCAC
(0 other experimental conditions)
Note: This is a non-specific herpesvirus primer.
Reverse(POL2) primer
Oligo type: Reverse PrimerDegeneracy: 2
Target: 21646-21630Tm: 64.6C at salt 1000.0 mMLength:
Sequence: ATT(A/G)TCCACAAACTGTTTTGT
(2 other experimental conditions)
Note: This is a non-specific herpesvirus primer.
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