A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SA0001Publication date: 2004
PMID: 15652214GI: 9627521 Taxonomy ID: 10255
Virus Name: Variola virusProduct size (PCR): 203 bp
MgCl2: 2 mMdNTPs: mMType: Multiplex PCR
Temp (PCR cycle):93C 120sec>(93C 30sec>50C 45sec>72C 120sec)*30>72C 600sec
Buffer: 60mM Tris–HCl pH 8.5, 25mM KCl, 10mM 2-mercaptoethanol, 0.1% Triton X-100
Polymerase: Taq polyCycler: GeneAmp PCR System 9700
Forward(VARV) primer
Oligo type: Forward PrimerDegeneracy: 1
Target: 7685-7710Tm: 72.6C at salt 1000.0 mMLength:
Sequence: catccgatattattgtaaccacaatg
(0 other experimental conditions)
Note: 1150658
Reverse(VARVR) primer
Oligo type: Reverse PrimerDegeneracy: 1
Target: 7887-7864Tm: 73.6C at salt 1000.0 mMLength:
Sequence: cccaacatttctaaatctcctcgt
(0 other experimental conditions)
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