A Simple Solution for Oligonucleotide Design

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2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP1093Publication date: 06/03
PMID: 12765993GI: 30314341 Taxonomy ID: 227859
Virus Name: SARS coronavirus, SARSProduct size (PCR): 182 bp
MgCl2: 3.5 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):95C 10min>(95C 10sec>57C 5sec>72C 9sec)*50
Buffer: N/A
Polymerase: N/ACycler: LightCycler (Roche)
Note: RT was performed in a total volume of 20microL including 10microL RNA, 10U/microL Superscript II RTase, 7.5microg/L random hexamers, 10mM dithiothreitol, and 0.5mM dNTPs.

PCR was performed in a total volume of 20microL with FastStart DNA Master SYBR Green I florescence reaction (Roche) and 12.5microM each primer.
Reverse(coro4) primer
Oligo type: PCRDegeneracy: 1
Target: 506-491Tm: 48 CLength:
Sequence: CACGAACGTGACGAAT
(3 other experimental conditions)
Note:
Forward(coro3) primer
Oligo type: PCRDegeneracy: 1
Target: 325-341Tm: 52 CLength:
Sequence: TACACACCTCAGCGTTG
(2 other experimental conditions)
Note:
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