A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0915Publication date: 09/02
PMID: 12202582GI: 22770295 Taxonomy ID: 35252
Virus Name: Alcelaphine herpesvirus 1, AlHV-1; Alcelaphine herpesvirus 2, AlHV-2Product size (PCR):
bp
MgCl2: 2.5 mMdNTPs: mMType: Nested PCR
Temp (PCR cycle):(94C 30sec>47C 30sec>72C 20sec)*30>72C 7min
Buffer: 15mM Tris (pH 8.0), 50mM KCl
Polymerase: 0.05U/microL AmpliTaq Gold DNA poly (Applied Biosystems)Cycler: N/A
Note: Primers amplify both:
Alcelaphine herpesvirus 1 [Taxonomy:35252]
Alcelaphine herpesvirus 2 [Taxonomy:138184]

External Nested reaction was performed using using the same materials and primers as in Lehijani et al., (1994) [PMID:7858018].
1microL of the external reaction was used as a template in the internal reaction.

Other PCRs in this article did not give the oligonucleotides used or did not amplify viruses.
Forward(PM00578) primer
Oligo type: Degeneracy: 1
Target: 19-37Tm: 65 C at salt 1000.0mMLength:
Sequence: TTTATTGAAGAAGTGGCTC
(0 other experimental conditions)
Note:
Reverse(PM00579) primer
Oligo type: Degeneracy: 1
Target: 134-117Tm: 50 CLength:
Sequence: CCATTTTGTTTTGTCCTG
(0 other experimental conditions)
Note:
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.