A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0440Publication date: 08/95
PMID: 7559950GI: 335637 Taxonomy ID: 10242
Virus Name: Vaccinia virus, VAC; Rabbitpox virus, RPV; Buffalopox virus, BFL; Ectromelia virus, ECT; Camelpox virus, CML; Taterapox virus, GBL; Cowpox virus, CPV; Variola virus, VAR; Monkeypox virus, MPVProduct size (PCR): N/A bp
MgCl2: 2.5 mMdNTPs: mMType: Universal PCR
Temp (PCR cycle):(94C 1min>55C 2min>72C 3min)*25
Buffer: 50mM KCl, 10mM Tris-HCl (pH 8.3)
Polymerase: 0.025U/microL DNA poly (Perkin-Elmer Cetus Corp., Norwalk, Conn.)Cycler: Model 9600 thermal cycler (Perkin-Elmer Cetus)
Note: Primers amplify the following viruses:
Vaccinia virus [Taxonomy:10245]
Rabbitpox virus [Taxonomy:32606]
Buffalopox virus [Taxonomy:32605]
Ectromelia virus [Taxonomy:12643]
Camelpox virus [Taxonomy:28873]
Taterapox virus [Taxonomy:28871]
Cowpox virus [Taxonomy:10243]
Variola virus [Taxonomy:10255]
Monkeypox virus [Taxonomy:10244]

PCR uses 5microg/mL of each primer and 0.5ng/microL template DNA in a total volume of 100microL.
For the other virus specific PCRs in this article see: [see MP0439], [see MP0441], [see MP0442], [see MP0443], [see MP0444], [see MP0445], [see MP0446], [see MP0447], [see MP0448], [see MP0449], [see MP0450]
Forward(EACP1) primer
Oligo type: Degeneracy: 1
Target: 192-210Tm: 67.2 C at salt 1000.0mMLength:
Sequence: ATGACACGATTGCCAATAC
(1 other experimental conditions)
Note:
Reverse(EACP2) primer
Oligo type: Degeneracy: 1
Target: 1136-1120Tm: 46 CLength:
Sequence: CTAGACTTTGTTTTCTG
(1 other experimental conditions)
Note:
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