A Simple Solution for Oligonucleotide Design

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Oligo Sequence:

°C± °C
Oligo length:
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Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0265Publication date: 04/97
PMID: 9129644GI: 7960753 Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR): 738 bp
MgCl2: 2.5 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):(94C 1min>52C 1min>72C 1min)*35>72C 7min
Buffer: 1x PCR buffer (10mM Tris-HCl, pH 9.0, 50mM KCl, 0.1mg/ml BSA)
Polymerase: 0.02U/microL Taq poly (Perkin Elmer-Cetus)Cycler: Perkin Elmer Cetus Thermocycler
Note: RT procedure- Mixture for cDNA synthesis consisted of 4microL extracted RNA, 0.0008U/microL random Hexamers (Pharmacia). Solution incubated at 65C 5min. Then 0.96U/microL RNAguard (Pharmacia), 5microL 1st strand reaction buffer(Gibco BRL), 0.2mM dNTP (Pharmacia), 8U/microL Moloney murine leukaemia virus reverse transcriptase (Gibco). Reaction then incubated at 37C 90min>98C 5min.
This article contained two PCRs,
NC region: [see MP0264]
p20 region: [see MP0265]
Forward(BD1) primer
Oligo type: PCRDegeneracy: 1
Target: 367-388Tm: 74.7 C at salt 1000.0mMLength:
(0 other experimental conditions)
Reverse(BD2) primer
Oligo type: PCRDegeneracy: 8
Target: 1104-1085Tm: Max Tm 74.1 C Min Tm 67.9 C at salt 1000.0mMLength:
(1 other experimental conditions)
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