A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0258Publication date: 10/00
PMID: 11131328GI: 9629506 Taxonomy ID: 31656
Virus Name: bovine viral diarrhea virus (strain SD-1), BVDVProduct size (PCR): 213 bp
MgCl2: 4 mMdNTPs: mMType: RT- Nested PCR 2
Temp (PCR cycle):(94C 1min>55C 1min>72C 45 sec)*30>72C 10min
Buffer: Same as first PCR
Polymerase: 0.61 U/microL Taq poly (Promega, Madison, WI)Cycler: N/A
Note: This is a one tube RT-Nested PCR.
The materials for this PCR were stored in the lid of the tube.
For external PCR and materials [see MP0257]
Forward(BVD 180) primer
Oligo type: Degeneracy: 3
Target: 176-197Tm: Max Tm 78.5 C Min Tm 76.6 C at salt 1000.0mMLength:
Sequence: CCTGAGTACAGGG(A/G/T)AGTCGTCA
(1 other experimental conditions)
Note: Used with HCV 368 to amplify a 213bp product.
Reverse(HCV 368) primer
Oligo type: Degeneracy: 1
Target: 389-373Tm: 52 CLength:
Sequence: CCATGTGCCATGTACAG
(4 other experimental conditions)
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.
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