A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0142Publication date: 1999
PMID: 10403696GI: 7960753 Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR): 372 bp
MgCl2: N/A mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):(94C 1min>56C 1min>72C 1min)*35
Buffer: N/A
Polymerase: N/ACycler: N/A
Note: RT: Synthesis of cDNA was carried out using random hexamers and Moloney murine leukaemia virus reverse transcriptase.
RT-PCR performed as in:(PMID: 8031236) Vilcek S., Herring A.J., Herring J.A., Nettleton P.F., Lowings J.P., and Paton D.J., Arch Virol 136, 309-323, 1994.
Forward(B11) primer
Oligo type: PCRDegeneracy: 4
Target: 12174-12201Tm: Max Tm 74.7 C Min Tm 73.3 C at salt 1000.0mMLength:
Sequence: ACATACCTGA(C/T)AAAGGCT(A/T)TACATTACA
(0 other experimental conditions)
Note: Target area found in article
Reverse(B12) primer
Oligo type: PCRDegeneracy: 4
Target: 12545-12526Tm: Max Tm 76.1 C Min Tm 74.1 C at salt 1000.0mMLength:
Sequence: TAGACGTCGGG(C/T)GT(A/T)TCCTC
(0 other experimental conditions)
Note: Target area found in article
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