A Simple Solution for Oligonucleotide Design

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Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0112Publication date: 10/94
PMID: 7597858GI: 9626649 Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR): 672 bp
MgCl2: 1.5 mMdNTPs: mMType: RT-Nested PCR 2
RT-Nested PCR 1
Temp (PCR cycle):94C 45sec>(94C 45sec>55C 45sec[+2sec/cyc]>72C 30sec[+5sec/cyc])*30>72C 10min
Buffer: 50mM KCl, 10mM Tris-HCl (pH 9.0), 0.01% gelatin, 0.1% Triton X-100
Polymerase: 0.01U/microL Tth polyCycler:
Note: For RT and primary PCR, [see MP0111].

Total PCR volume is 50microL containing 2microL primary PCR product and 1microM each primer.
Polymerase was added at 70C after the first denaturation step.

Other strains of BVDV produce a 402bp product.

For other PCR in this article, [see MP0110].
Forward(MP01101) primer
Oligo type: Degeneracy: 1
Target: 4934-4956Tm: 69.7 C at salt 1000.0mMLength:
(2 other experimental conditions)
Reverse(MP01102) primer
Oligo type: Degeneracy: 1
Target: 5605-5588Tm: 54 CLength:
(3 other experimental conditions)
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