A Simple Solution for Oligonucleotide Design

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Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0110Publication date: 10/94
PMID: 7597858GI: 9626649 Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR): 672 bp
MgCl2: 1.5 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):94C 45sec>(94C 45sec>55C 45sec[+2sec/cyc]>72C 30sec[+5sec/cyc])*35>72C 10min
Buffer: 50mM KCl, 10mM Tris-HCl (pH 9.0), 0.01% gelatin, 0.1% Triton X-100
Polymerase: 0.01U/microL Tth polyCycler:
Note: This single-step RT-PCR is performed as described by Gruber et al, (1993) [PMID:8408445].

Other strains of BVDV produce a 402bp product.

For other PCRs in this article, [see MP0111] and [see MP0112].
Forward(MP01101) primer
Oligo type: PCRDegeneracy: 1
Target: 4934-4956Tm: 69.7 C at salt 1000.0mMLength:
(2 other experimental conditions)
Reverse(MP01102) primer
Oligo type: PCRDegeneracy: 1
Target: 5605-5588Tm: 54 CLength:
(3 other experimental conditions)
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