A Simple Solution for Oligonucleotide Design

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Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0082Publication date: 01/94
PMID: 8011781GI: 9626649 Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR): N/A bp
MgCl2: 1.4 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):(95C 1min>55C 1min>72C 2min)*40
Buffer: 40mM Tris-HCl, 60mM KCl, 1% gelatin
Polymerase: 0.5U/microL Taq poly (Promega, Madison, WI.)Cycler: N/A
Note: 5microL of the RT reaction was used for PCR.
RT procedure - materials 40microM of primer MP00821, 40microM of primer MP00822, 7.5microM dNTP's, Schimke solution(4mM dithiothreitol, 4mM MgCl2, 280mM KCl, 32mM Tris; pH 8), 19.6U/ml reverse transcriptase (Life Sciences, St. Petersburg, Fl.), 28U/microL RNase inhibitor (Pharmacia, Alameda, CA). RT reaction mixture was incubated at 42C for 2hr.
Forward(MP00821) primer
Oligo type: PCRDegeneracy: 1
Target: 188-205Tm: 56 CLength:
Sequence: GTAGTCGTCAGTGGTTCG
(3 other experimental conditions)
Note:
Reverse(MP00822) primer
Oligo type: PCRDegeneracy: 1
Target: 383-366Tm: 54 CLength:
Sequence: GCCATGTACAGCAGAGAT
(3 other experimental conditions)
Note:
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