A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0080Publication date: 08/91
PMID: 1656820GI: N/A Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR): 562 bp
MgCl2: 2 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):94C 1min>37C 30sec>72C 10min>(94C 1min>37C 30sec>72C 30sec)*5>(94C 1min>55C 30sec>72C 30sec)*30
Buffer: 1xPCR buffer (50mM KCl, 10mM Tris, 0.01% gelatin, pH8.8)
Polymerase: 0.025U/microL Taq 1 poly (Amplitaq, Cetus Corp, Emeryville, Calif.)Cycler: Thermocycler (Perkin-Elmer/Cerus Corp. Norwalk, Conn.)
Note: RT procedure - materials: 200microM dNTP, 50pmol DNA hexamers, 0.125U/microL of Avian myeloblastosis virus reverse transcriptase, 1xPCR buffer, 2mM MgCl2. Mixture was then incubated at 22C 10min>43C 120min>95C 5min> then quickly cooled.
Forward(p80.1) primer
Oligo type: PCRDegeneracy: 32
Target: N/A-N/ATm: Max Tm 85.1 C Min Tm 79 C at salt 1000.0mMLength:
Sequence: TCGAGCTCGGATGAA(A/G)GG(A/C/G/T)GA(A/G)GA(T/C)CT
(0 other experimental conditions)
Note:
Reverse(p80.2) primer
Oligo type: PCRDegeneracy: 16
Target: N/A-N/ATm: Max Tm 82 C Min Tm 76.2 C at salt 1000.0mMLength:
Sequence: GCTCTAGAGCCCA(T/G)TC(A/G)TA(A/C)TTCAT(T/C)TC
(0 other experimental conditions)
Note:
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