A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0071Publication date: 07/92
PMID: 1322931GI: 323205 Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR):
bp
MgCl2: 1.44 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):94C 2min>(94C 1min>55C 1.5min>72C 3min)*30>72C 7min
Buffer: 1x PCR buffer (50mM KCl, 10mM Tris-HCl pH 8.3)
Polymerase: 0.025U/microL Amplitaq poly (Perkin-Elmer Cetus Corp., Norwalk, CT)Cycler: N/A
Note: RT procedure- reaction contained 1x RT buffer(10mM Tris-HCl pH 8.3, 8 mMKCl, 1.2mM MgCl2, 0.1 mg/ml BSA), 1mM dNTP, 0.625U/microL RNasin (Promega Corp.), 20ng/microL random hexanucleotides, and 10U/microL M-MLV reverse transcriptase. Mixture was incubated at 37C 45min>95C 5min.
Reverse(12434) primer
Oligo type: PCRDegeneracy: 1
Target: 12451-12434Tm: 60 CLength:
Sequence: ACGACACGCCCAACACGC
(0 other experimental conditions)
Note:
Forward(231) primer
Oligo type: PCRDegeneracy: 1
Target: 231-249Tm: 78 C at salt 1000.0mMLength:
Sequence: TGCCACGTGGACGAGGGCA
(0 other experimental conditions)
Note:
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