A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0053Publication date: 02/95
PMID: 7714180GI: 323205 Taxonomy ID: 11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDVProduct size (PCR): 244 bp
MgCl2: 1.5 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):42C 30min>95C 3min>(95C 30sec>58C 15sec>72C 30sec)*35>72C 10min
Buffer: 50mM KCl, 10mM Tris pH 9.0, 0.1% Triton X-100, 0.04U/mivroL RNase inhibitor (Professional Diagnostics, Edmonton, Alberta, Canada)
Polymerase: 0.01U/microL Taq poly (Fisher Scientific)Cycler: MJR model PTC100-60 (Fisher Scientific)
Note: One tube RT-PCR
Forward(BVD-99) primer
Oligo type: PCRDegeneracy: 1
Target: 99-118Tm: 74.1 C at salt 1000.0mMLength:
(0 other experimental conditions)
Forward(BVD-342) primer
Oligo type: PCRDegeneracy: 1
Target: 342-324Tm: 73.7 C at salt 1000.0mMLength:
(0 other experimental conditions)
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