A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0026Publication date: 11/96
PMID: 9055637GI: 330745 Taxonomy ID: 10320
Virus Name: Bovine herpesvirus 1, BHV-1Product size (PCR): 381 bp
MgCl2: N/A mMdNTPs: mMType: PCR
Temp (PCR cycle):(95C 1min>57C 2min>72C 1min)*5>(95C 40sec>57C 40sec>72C 40sec)*30
Buffer: 1x reaction Buffer
Polymerase: 0.02U/microL Taq poly (Bangalore Genie)Cycler: Perkin-Elmer-Cetus DNA thermal cycler
Note:
Forward(P1) primer
Oligo type: PCRDegeneracy: 1
Target: 311-330Tm: 76.1 C at salt 1000.0mMLength:
Sequence: ACATGCTGGCGCTGATCGCA
(0 other experimental conditions)
Note: The article listed the sequence at 227-246 but the nucleotide sequence database used had it at 311-330.
Reverse(P2) primer
Oligo type: PCRDegeneracy: 1
Target: 691-672Tm: 78.5 C at salt 1000.0mMLength:
Sequence: CGAGTTTCGAGACCAGCAGTCC
(0 other experimental conditions)
Note: The article listed the location of this sequence at 585-607 but the GI# databse had it at 691-672
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