A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0009Publication date: 06/95
PMID: 8701569GI: 1491620 Taxonomy ID: 10320
Virus Name: Bovine herpesvirus 1, BHV-1Product size (PCR): 468 bp
MgCl2: N/A mMdNTPs: mMType: PCR
Temp (PCR cycle):(95C 1min>56C 1min>72C 1min)*35
Buffer: 2.5 microL 10x reaction buffer (Boehringer-Mannheim)
Polymerase: 0.5U/microL Taq poly (Boehringer-Mannheim)Cycler: Perkin-Elmer-Cetus DNA thermal cycler
Note: Procedure in detail in:(PMID: 8388400) Vilcek,S. (1993). Detection of bovine herpesvirus-1 (BHV-1) genome by PCR. J. Virol. Meth., 41: 245-248
Forward(P1) primer
Oligo type: PCRDegeneracy: 1
Target: 624-645Tm: 82.1 C at salt 1000.0mMLength:
Sequence: CACGGACCTGGTGGTGGACAAGAAG
(0 other experimental conditions)
Note: The target start and end in the article was not found in the GI found but an identicle series was found at 25217-25241
Reverse(P2) primer
Oligo type: PCRDegeneracy: 1
Target: 1091-1070Tm: 78.5 C at salt 1000.0mMLength:
Sequence: CTACCGTCACGTGCTGTGTACG
(2 other experimental conditions)
Note: This sequence did not match the sequence in the GI number and couldn't be found in a bovine herpesvirus
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.