A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MP0008Publication date: 08/95
PMID: 8861645GI: 330756 Taxonomy ID: 10323
Virus Name: Bovine herpesvirus 1.1 (strain Cooper), BHV-1.1Product size (PCR): 323 bp
MgCl2: 1.5 mMdNTPs: mMType: PCR
Temp (PCR cycle):(94C 30sec>58C 1min>72C 1min)*45>72C 10min
Buffer: 10mM Tris-HCl pH 8.3, 50mM KCL, 0.01%gelatine
Polymerase: 0.03U/microL Taq poly (Cetus, PerkinElmer)Cycler:
Note:
Forward(IB1) primer
Oligo type: PCRDegeneracy: 1
Target: 1491-1508Tm: 50 CLength:
Sequence: AAGCGCAAAAACGTGTG
(0 other experimental conditions)
Note:
Reverse(IB3) primer
Oligo type: PCRDegeneracy: 1
Target: 1814-1798Tm: 54 CLength:
Sequence: TGCAGGTACAGCTTGGC
(0 other experimental conditions)
Note:
Forward(IB0) primer
Oligo type: hybridizationDegeneracy: 1
Target: 1611-1640Tm: 86.1 C at salt 1000.0mMLength:
Sequence: AGCCACACCTTCGCGTTGCAGAATGTGCCG
(0 other experimental conditions)
Note: Designed for Southern blot hybridization, labled with digoxigenin (DIG) 3' end labelling kit, Hybridization carried out overnight at 68c in same buffer as experiment with 4pmol/ml of DIG-labelled probe
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