A Simple Solution for Oligonucleotide Design

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2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MO0005Publication date: 01/99
PMID: 10028170GI:
Taxonomy ID: 144353
Virus Name: bovine viral diarrhea virus, BVDV-1, BVDV-2Product size (PCR): 221 bp
MgCl2: 3 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):95C 5min>(94C 1min>52C 1min>72C 1min)*35
Buffer: 20mM Tris-HCl (pH 8.4); 50mM KCl
Polymerase: 0.05U/microL Taq poly (GIBCO BRL)Cycler:
Note: For B3/B4 and B5/B6 primer pairs.The B3 and B4 amplify BVDV type 1 and B5 and B6 amplify BVDV type 2.
The GI number was too hard to find.
Forward(B3) primer
Oligo type: PCRDegeneracy: 1
Target: 139-155Tm: 54 CLength:
Sequence: GGTAGCAACAGGGTGAG
(0 other experimental conditions)
Note: This primer is for BVDV type 1. The target area was specified in the article and was only for 16 base pairs.
Reverse(B4) primer
Oligo type: PCRDegeneracy: 1
Target: 360-343Tm: 56 CLength:
Sequence: GTAGCAATACAGTGGGCC
(0 other experimental conditions)
Note: This primer is for BVDV type one. The target area was specified in the article and only for 17 base pairs
Forward(B5) primer
Oligo type: PCRDegeneracy: 1
Target: 139-145Tm: 56 CLength:
Sequence: ACTAGCGGTAGCAGTGAG
(0 other experimental conditions)
Note: This primer for BVDV type 2. The target area was specified in the article and is only for 6 base pairs.
Forward(B6) primer
Oligo type: PCRDegeneracy: 1
Target: 360-343Tm: 56 CLength:
Sequence: CTAGCGGAATAGCAGGTC
(0 other experimental conditions)
Note: This primer was for BVDV type 2. The target area was specified in the article and is only for 17 base pairs.
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