A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: MO0002Publication date: 08/98
PMID: 9810617GI: 330756 Taxonomy ID: 10320
Virus Name: Bovine herpesvirus 1, BHV-1Product size (PCR): 344 bp
MgCl2: 2.5 mMdNTPs: mMType: Nested PCR 2
NESTED PCR 1
Temp (PCR cycle):(95C 60sec>56C 60sec>72C 60sec)*35>72C 6min
Buffer: 10mM Tris-HCl pH 8.3; 50mM KCl; 5% glycerol
Polymerase: 1.0U/microL Taq polyCycler:
Note: The concentration of MgCl2 used was from 1.0 to 4.0 mM.
Forward(MO00021) primer
Oligo type: PCRDegeneracy: 1
Target: 1046-1063Tm: 60 CLength:
Sequence: AGCCGAGTACCTGCGCAG
(2 other experimental conditions)
Note:
Reverse(MO00022) primer
Oligo type: PCRDegeneracy: 1
Target: 1389-1372Tm: 58 CLength:
Sequence: AGCCCTCGATCTGCTGGA
(2 other experimental conditions)
Note:
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