A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: JX0068Publication date: 03/97
PMID: 9079767GI: 1916855 Taxonomy ID: 10255
Virus Name: Variola virus; Monkeypox virus; Cowpox virus; Vaccinia virus (strain WR); Ectromelia virus; camelpox virus; volepox virus; Raccoonpox virus; skunkpox virusProduct size (PCR):
bp
MgCl2: 2.5 mMdNTPs: mMType: ATI-PCR
Temp (PCR cycle):(94C 1min>40C 1min>72C 2.5min)*25>72C 10min
Buffer: 50mM KCl, 10mM Tris-HCl pH 8.3
Polymerase: 0.05U/microL Taq poly (Boehringer-GmbH, Germany)Cycler: GeneAmp PCR System 9600 (Perkin-Elmer Cetus, Norwalk, CT)
Note: ATI PCR is the universal PCR using a single primer pair based on sequences coding for the major protein component of the cowpox virus acidophilic-type inclusion body. This PCR can be used to identify and differentiated the orthopoxvirus species.
Primer ATI-up-1 and ATI-low-1 were used to amplify the following organisms:
Variola virus, [Taxonomy:10255]
Monkeypox virus, [Taxonomy:10244]
Cowpox virus, [Taxonomy:10243]
Vaccinia virus (strain WR), [Taxonomy:10254]
Ectromelia virus, [Taxonomy:12643]
camelpox virus, [Taxonomy:28873]
Raccoonpox virus, [Taxonomy:10256]
Total PCR volume is 50microL containing 5ng/microL of DNA and 2ng/microL of each primer.
Forward(ATI-up-1) primer
Oligo type: Degeneracy: 1
Target: 1715-1730Tm: 44 CLength:
Sequence: AATACAAGGAGGATCT
(2 other experimental conditions)
Note:
Reverse(ATI-low-1) primer
Oligo type: Degeneracy: 1
Target: 2780-2763Tm: 46 CLength:
Sequence: CTTAACTTTTTCTTTCTC
(2 other experimental conditions)
Note:
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