A Simple Solution for Oligonucleotide Design

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Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: JX0066Publication date: 01/00
PMID: 10644091GI: 5921457 Taxonomy ID: 12115
Virus Name: Foot-and-mouth disease virus (strain A24 Cruzeir)Product size (PCR): 131 bp
MgCl2: 4.6 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):(95C 10sec>60C 10sec)*5>(95C 0sec>60C 0sec>72C 5sec)*25
Buffer: 18mM Tris-HCl (pH 8.3), 23mM KCl
Polymerase: 0.5microL Stoffel fragment of Taq poly (Perkin-Elmer)Cycler: Perkin-Elmer 9600
Note: Total RT volume is 10microL containing 50mM Tris-HCl (pH 8.3), 75mM KCl, 3mM MgCl2, 0.01M dithiothreitol, 1 mM each dNTP, 0.5microM random hexamers (Gibco), 0.01U/microL RNAsin (Promega), and 2U/microL superscript II RTase.
RT temperature: 41C 45min>95C 8min>10C 3min.

Total PCR volume is 50microL containing all 10microL cDNA and 0.3microM each primers PD00385 and PD00386.
Article only gives Taq in volume used.

For other PCR in this article, [see JX0067].
Reverse(PD00385) primer
Oligo type: Degeneracy: 1
Target: 3711-3692Tm: 74.1 C at salt 1000.0mMLength:
Sequence: AGCTTGTACCAGGGTTTGGC
(11 other experimental conditions)
Note:
Forward(PD00386) primer
Oligo type: Degeneracy: 1
Target: 3940-3959Tm: 74.1 C at salt 1000.0mMLength:
Sequence: CAGATGCAGGAGGACATGTC
(3 other experimental conditions)
Note:
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