A Simple Solution for Oligonucleotide Design

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2. Search by PMID, VirOligoID, or taxonomy ID;

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Oligo Sequence:

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Oligo length:
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GI Number:

CommonData ID: JX0031Publication date: 03/01
PMID: 11164915GI: 61076 Taxonomy ID: 73482
Virus Name: Foot-and-mouth disease virus (strain O1)Product size (PCR):
MgCl2: 2.2 mMdNTPs: mMType: Real-Time PCR
Temp (PCR cycle):50C 2min>95C 10min>(95C 15sec>60C 60sec)*50
Buffer: 1x TaqMan master mix (PE Applied Biosystems, UK)
Polymerase: Taq Gold thermostable poly (PE Applied Biosystems)Cycler: PE Applied Biosystems 5700 thermal cycler (PE Applied Biosystems, UK)
Note: Total RT volume is 10microL containing 3.85microL RNA, 50mM KCl, 10mM Tris-HCl (pH 8.3), 5.5mM MgCl2, 0.5 mM each dNTP, 2.5microM random hexamers, 0.4U/microL RNase inhibitos, 0.25U/microL MultiScribe Moloney RTase.
RT temperature: 48C 45min>95C 5min.

Total TaqMan PCR is 25microL containing 0.2microM probe SA-P-IR 110-131F and 0.9microM each primers SAIR 89F and SAIR 151R.
Article does not mention polymerase concentration for PCR.
For SYBR PCR [see JX0030].
Forward(SAIR 89F) primer
Oligo type: Degeneracy: 1
Target: 386-405Tm: 76.1 C at salt 1000.0mMLength:
(0 other experimental conditions)
Reverse(SAIR 151R) primer
Oligo type: Degeneracy: 1
Target: 448-433Tm: 56 CLength:
(0 other experimental conditions)
Forward(SA-P-IR 110-131F) primer
Oligo type: Degeneracy: 1
Target: 407-428Tm: 80.3 C at salt 1000.0mMLength:
(0 other experimental conditions)
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