A Simple Solution for Oligonucleotide Design

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CommonData ID: JP0039Publication date: 01/02
PMID: 11808702GI: 330752 Taxonomy ID: 10295
Virus Name: Bovine herpesvirus 2Product size (PCR):
MgCl2: 1.5 mMdNTPs: mMType: PCR
Temp (PCR cycle):94C 3min>(94C 1min>68C 1min>72C 1min)*40>72C 10min
Buffer: 1x PCR Buffer, 2% DMSO
Polymerase: 0.05U/microL Taq DNA polyCycler: PTC-100 thermal cycler (MJ Research, Waltham MA, USA)
Note: Total RT volume was 10 microL and contained 2.5U/microL MuLV RTase, 1U/microL RNase inhibitor, 100ng/microL primer P2, and 0.32mM dNTP. RT temperature: 42C 15min>99C 5min>4C 5min.

Total PCR volume was 50microL,and contained 20ng/microL of each primer.

For other PCR in this article; [see JP0038], [see JP0040], [see JP0041].
Forward(4109) primer
Oligo type: Degeneracy: 1
Target: 2416-2440Tm: 87 C at salt 1000.0mMLength:
(0 other experimental conditions)
Reverse(4110) primer
Oligo type: Degeneracy: 1
Target: 2837-2810Tm: 82 C at salt 1000.0mMLength:
(0 other experimental conditions)
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