A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: HI0001Publication date: 12/00
PMID: 11101621GI: 261087 Taxonomy ID: 10385
Virus Name: Bovine herpesvirus 4, BoHV-4Product size (PCR): 2538 bp
MgCl2: 3.0 mMdNTPs: mMType: PCR
Temp (PCR cycle):94C 5min>(94C 1min>55C 1min>72C 2min)*30>72C 5min
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl
Polymerase: 0.02U/microL Taq poly (Boehringer Diagnostics, Milan, Italy)Cycler:
Note: Final PCR volume is 50microL containing 0.25microM each primer and 1microL DNA.
Forward(alpha) primer
Oligo type: PCRDegeneracy: 1
Target: -1-24Tm: 72.3 C at salt 1000.0mMLength:
Sequence: CGAATTATAGTCTAAAGTCATCCTC
(1 other experimental conditions)
Note: First bp does not complement GI.
Mismatch at position 6.
Reverse(beta) primer
Oligo type: PCRDegeneracy: 1
Target: 1013-989Tm: 75.5 C at salt 1000.0mMLength:
Sequence: GTAAGGACCTTTCACACTCTTAAGC
(0 other experimental conditions)
Note: Multiple mismatches.
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