A Simple Solution for Oligonucleotide Design

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Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: CW0005Publication date: 09/97
PMID: 9261403GI: 9629818 Taxonomy ID: 10320
Virus Name: Bovine herpesvirus 1, BHV 1Product size (PCR):
bp
MgCl2: 1.5 mMdNTPs: mMType: PCR
Temp (PCR cycle):(95C 1min>65C 1min>72C 2min)*40
Buffer: 1x Commerical PCR buffer
Polymerase: 0.66 U/microL Taq polyCycler: N/A
Note: Contains 0.5 mM more MgCl2 than CW0004
contains 0.33 U/microL more Taq poly than CW0004
Forward(CW00050) primer
Oligo type: PCRDegeneracy: 1
Target: 113556-113574Tm: 82.3 C at salt 1000.0mMLength:
Sequence: GCGCTGGTCCTCCGGCTCC
(0 other experimental conditions)
Note: specific to bICP22 transcript
Reverse(CW00051) primer
Oligo type: PCRDegeneracy: 1
Target: 113665-113648Tm: 64 CLength:
Sequence: CTCGCTGGCGGCGCTTGG
(0 other experimental conditions)
Note: specific to bICP22 transcript
Forward(CW00052) primer
Oligo type: HybridizationDegeneracy: 1
Target: 113608-113626Tm: 84.4 C at salt 1000.0mMLength:
Sequence: GGCTCGCCCTCCGGTCGCG
(0 other experimental conditions)
Note: Hypbridization probe specific to bICP22 transcript
Pre-hybridization 63C 60 min in 1x SSPE (150 mM NaCl,10 mM NaH2PO4, 1mM EDTA; PH7), 0.2xBP (1XBP is 2% bovine serum albumin, 2% polyvinylpyrolidone) plus 1%SDS.
Hybridization 62C 180 min in 1x SSPE-0.2XBP-1%SDS
Post-Hybridization: Wash at room temperature for 5min in 0.005 SSPE-0.05%SDS three times.
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