A Simple Solution for Oligonucleotide Design

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1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: CW0004Publication date: 09/97
PMID: 9261403GI: 9629818 Taxonomy ID: 10320
Virus Name: Bovine herpesvirus 1, BHV 1Product size (PCR):
bp
MgCl2: 1.0 mMdNTPs: mMType: PCR
Temp (PCR cycle):(95C 1min>65C 1min>72C 2min)*40
Buffer: 1x Commerical PCR buffer
Polymerase: 0.33 U/microL Taq polyCycler: N/A
Note:
Forward(CW00040) primer
Oligo type: PCRDegeneracy: 1
Target: 101447-101466Tm: 80.2 C at salt 1000.0mMLength:
Sequence: TTCTCTGGGCTCGGGGCTGC
(1 other experimental conditions)
Note: specific to bICP0 transcript
Reverse(CW00041) primer
Oligo type: PCRDegeneracy: 1
Target: 101616-101638Tm: 80.4 C at salt 1000.0mMLength:
Sequence: AGAGGTCGACAAACACCCGCGGT
(1 other experimental conditions)
Note: specific to bICP0 transcript
Forward(CW00042) primer
Oligo type: HybridizationDegeneracy: 1
Target: 101577-101597Tm: 86.1 C at salt 1000.0mMLength:
Sequence: CCGCAAGGGCGGCGCGCTAGC
(0 other experimental conditions)
Note: Hybridization probe specific to bICP0 transcript
Pre-hybridization 63C 60 min in 1x SSPE (150 mM NaCl,10 mM NaH2PO4, 1mM EDTA; PH7), 0.2xBP (1XBP is 2% bovine serum albumin, 2% polyvinylpyrolidone) plus 1%SDS.
Hybridization 62C 180 min in 1x SSPE-0.2XBP-1%SDS
Post-Hybridization: Wash at room temperature for 5min in 0.005 SSPE-0.05%SDS three times.
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