A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: BM1300Publication date: 04/96
PMID: 8908748GI: 209811 Taxonomy ID: 10546
Virus Name: bovine adenovirus; goat adenovirus 1; porcine adenovirusProduct size (PCR): 301 bp
MgCl2: 1.5 mMdNTPs: mMType: Univesal PCR
Temp (PCR cycle):94C 10min>(94C 30sec>55C 30sec>72C 30sec)*35>72C 5min
Buffer: 50mM KCl, 10mM Tris-HCl (pH 9.0)
Polymerase: 0.02U/microL Taq DNA polyCycler: DNA thermal cycler (Perkin Elmer Cetus)
Note: Primers amplify the following adenoviruses:
bovine 1 [Taxonomy:10546]
bovine 2 [Taxonomy:114429]
bovine 3 [Taxonomy:10510]
bovine 4 [Taxonomy:70333]
bovine 5 [Taxonomy:111166]
bovine 6 [Taxonomy:111167]
bovine 7 [Taxonomy:10511]
bovine 8 [Taxonomy:120509]
bovine 10 [Taxonomy:39788]
goat 1 [Taxonomy:111168]
porcine 5 [Taxonomy:45370]
porcine 1 [Taxonomy:45368]
porcine 3 [Taxonomy:35265]

Total PCR volume is 50microL containing 1-5microL DNA and 1.0microM each primer.

PCR is also performed in a PDR 91 DNA reproducer (BLS Ltd; Hungary).
Forward(HexAdB) primer
Oligo type: Degeneracy: 4
Target: 18858-18882Tm: Max Tm 82.1 C Min Tm 78.8 C at salt 1000.0mMLength:
Sequence: GCCGCA(A/G)TGGTC(C/T)TACATGCACATC
(0 other experimental conditions)
Note:
Reverse(HexAdJ) primer
Oligo type: Degeneracy: 16
Target: 19158-19136Tm: Max Tm 82.2 C Min Tm 75 C at salt 1000.0mMLength:
Sequence: CAGC(A/G)(C/T)(A/G)CCGCGGATGTCAAA(A/G)T
(0 other experimental conditions)
Note:
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