A Simple Solution for Oligonucleotide Design

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2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: BM0306Publication date: 06/98
PMID: 9694330GI: 61076 Taxonomy ID: 12120
Virus Name: Foot-and-mouth disease virus (strain C1-Santa Pau), FMDV C-S8 Sp70; Foot-and-mouth disease virus (strain O1), FMDV O1 Kb; Foot-and-mouth disease virus (strain A5), FMDV A5 WwProduct size (PCR):
bp
MgCl2: 1.5 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):93C 10min>(93C 1min>37C 1min>72C 2min)*30
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl, 0.01% gelatine, 10mM DTT, 0.4U/microL ribonuclease inhibitor, 0.1U/microL AMV RTase
Polymerase: 0.025U/microL Taq Gold poly (Perkin-Elmer, Cetus)Cycler:
Note: FMDV C-S8 Sp70 [Taxonomy:12120]
FMDV O1 Kb [Taxonomy:73482]
FMDV A5 Ww [Taxonomy:12113]

Total RT volume is 100microL containing 10mM Tris-HCl (pH 8.3), 50mM KCl, 1.5mM MgCl2, 0.01% gelatine, 10mM DTT, 100mM dNTPs, 0.4U/microL human placenta ribonuclease inhibitor, 2ng/microL primer 3D2, and 0.1U/microL AMV RTase (Seikagaku America).

After 42C for 40min, PCR is performed by adding 2ng/microL primer 3D-1 and polymerase to the reaction.

For other RT-PCRs in this article, [see BM0305], [see BM0307], [see BM0308], and [see BM0309].
Forward(3D1) primer
Oligo type: Degeneracy: 1
Target: 6317-6336Tm: 76.1 C at salt 1000.0mMLength:
Sequence: CAGAGATGTGGAAGAGCGCG
(2 other experimental conditions)
Note:
Reverse(3D2) primer
Oligo type: Degeneracy: 1
Target: 6524-6506Tm: 75.8 C at salt 1000.0mMLength:
Sequence: GCGGAACAGCGCTTTGTCC
(2 other experimental conditions)
Note:
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