A Simple Solution for Oligonucleotide Design

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Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: BM0041Publication date: 03/99
PMID: 10359157GI: 330756 Taxonomy ID: 10320
Virus Name: Bovine herpesvirus 1, BHV-1Product size (PCR):
bp
MgCl2: 1.5 mMdNTPs: mMType: Nested PCR 2
Nested PCR 1
Temp (PCR cycle):(95C 60sec>57C 60sec>72C 60sec)*35>72C 5min
Buffer: 10mM Tris-HCl (pH 8.3) 50mM KCl, 5% (w/v) glycerol
Polymerase: 0.05U/microL Taq DNA polyCycler:
Note: 2microL primary PCR product and 1mM each internal primer are used in a total PCR volume of 20microL. Prior to the addition of Taq and dNTPs, the samples were heated to 98C for 6min. For primary PCR, [see BM0040].
Article says that the reaction mixture contains 200mM dNTPs.
Forward(Int F) primer
Oligo type: Degeneracy: 1
Target: 1046-1063Tm: 60 CLength:
Sequence: AGCCGAGTACCTGCGCAG
(2 other experimental conditions)
Note: Specific to gp I glycoprotein region.
Article gives target as 663-680.
Reverse(Int R) primer
Oligo type: Degeneracy: 1
Target: 1389-1372Tm: 58 CLength:
Sequence: AGCCCTCGATCTGCTGGA
(2 other experimental conditions)
Note: Specific to gp I glycoprotein region.
Article gives target as 1007-990.
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