A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: BH0053Publication date: 01/91
PMID: 1845884GI: 291535 Taxonomy ID: 31518
Virus Name: Bovine herpesvirus type 1.1 (strain Jura); Bovine herpesvirus type 1.2 (strain K22)Product size (PCR):
bp
MgCl2: 10.0 mMdNTPs: mMType: Primer extension
Temp (PCR cycle):42C 2hr
Buffer: 1x AMV RTase buffer
Polymerase: 0.2UmicroL AMV RTaseCycler:
Note: Primer can extend the following bovine herpesviruses:
type 1.1 strain Jura [Taxonomy:31518]
type 1.2 strain K22 [Taxonomy:31519]

10microL containing 8x10^4 cpm labeled primer, 0.001mg/microL total RNA, 400mM NaCl, 1mM EDTA, and 40mM PIPES (pH 6.4).
Hybridization temperature: 45C 1hr.
A 40microL mixture containing 0.25U/microL AMV RTase, 1.25x AMV RTase buffer, and 0.625mM each dNTP is added to the RNA-primer mixture to create a total volume of 50microL.
Reverse(o3) primer
Oligo type: OtherDegeneracy: 1
Target: 2586-2568Tm: 75.8 C at salt 1000.0mMLength:
Sequence: TTGGCCGTCTCCTCCTTCC
(0 other experimental conditions)
Note:
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