A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: BH0003Publication date: 1990
PMID: 2241575GI: 10140926 Taxonomy ID: 35252
Virus Name: Alcelaphine herpesvirus 1, AHV-1Product size (PCR): 1028 bp
MgCl2: 1.5 mMdNTPs: mMType: PCR
Temp (PCR cycle):(92C 1min>62C 1min>72C 2min)*30
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl
Polymerase: 0.5U Taq polyCycler:
Note: Article does not mention total PCR volume.
PCR is performed using 1.0microM each primer.
Reverse(PN00065) primer
Oligo type: Degeneracy: 1
Target: 40349-40320Tm: 75.2 C at salt 1000.0mMLength:
Sequence: GTACACGTCCTTATATCTGTTATAAGTAGT
(0 other experimental conditions)
Note:
Forward(PN00066) primer
Oligo type: Degeneracy: 1
Target: 39321-39350Tm: 79.3 C at salt 1000.0mMLength:
Sequence: GATCTGTTAAGAAGGACTCTGAAATGCAGC
(0 other experimental conditions)
Note:
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