A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(PN00395) primer
Oligo ID:PN00395
Oligo type: Denegeracy: 2
Target: 11117-11096 Tm: Max Tm 72.9 C Min Tm 71 C at salt 1000.0mMLength: 22
Sequence: GCTGTTTCACCCAGTT(A/G)TACAT
(1 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: SK0102 Publication date: 06/99
PMID: 10325368 GI: 323205 Taxonomy ID:11099
Virus Name: pestivirus type 1, BVDV1; Bovine viral diarrhea virus genotype 2, BVDV2 Product size (PCR):
bp
MgCl2: 2.0 mM dNTPs: 0.2 mM Type: RT-Nested PCR
Temp (PCR cycle):(94C 20sec>50C 30sec>72C 30sec)*40>72C 15min
Buffer: 20mM Tris-HCl (pH 8.4), 50mM KCl
Polymerase: 0.025U/microL Taq DNA poly (Canadian Life Technologies)Cycler:
Note:



Oligonucleotide information
Reverse(PD00083) primer
Oligo ID:PD00083
Oligo type: Denegeracy: 2
Target: 11117-11096 Tm: Max Tm 72.9 C Min Tm 71 C at salt 1000.0mMLength: 22
Sequence: GCTGTTTCACCCAGTT(A/G)TACAT
(1 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP0300 Publication date: 06/99
PMID: 10325368 GI: 289507 Taxonomy ID:144353
Virus Name: bovine viral diarrhea virus, Bovine viral diarrhea virus-1 (Strain Singer), bovine viral diarrhea virus type 2 (Strain 890) Product size (PCR): N/A bp
MgCl2: 2 mM dNTPs: 0.2 mM Type: RT-Nested Multiplex PCR 2
Temp (PCR cycle):(94C 20sec>50C 30sec>72C 30sec)*40>72C 15min
Buffer: 20mM Tris-HCl [pH 8.4], 50mM KCl
Polymerase: 0.025U/microL Taq DNA poly (Canadian Life Technologies)Cycler: N/A
Note:

Total 2 Oligos
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