A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(MP00822) primer
Oligo ID:MP00821
Oligo type: PCR Denegeracy: 1
Target: 383-366 Tm: 54 CLength: 18
Sequence: GCCATGTACAGCAGAGAT
(3 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP0082 Publication date: 01/94
PMID: 8011781 GI: 9626649 Taxonomy ID:11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDV Product size (PCR): N/A bp
MgCl2: 1.4 mM dNTPs: 1.18 mM Type: RT-PCR
Temp (PCR cycle):(95C 1min>55C 1min>72C 2min)*40
Buffer: 40mM Tris-HCl, 60mM KCl, 1% gelatin
Polymerase: 0.5U/microL Taq poly (Promega, Madison, WI.)Cycler: N/A
Note:



Oligonucleotide information
Reverse(MP00892) primer
Oligo ID:MP00891
Oligo type: PCR Denegeracy: 1
Target: 383-366 Tm: 54 CLength: 18
Sequence: GCCATGTACAGCAGAGAT
(3 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP0089 Publication date: 11/94
PMID: 7975238 GI: 9626649 Taxonomy ID:11100
Virus Name: bovine viral diarrhea virus (isolate NADL); bovine viral diarrhea virus strain Oregon C24V; bovine viral diarrhea virus (strain SD-1); Bovine viral diarrhea virus genotype 2, BVDV II Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: RT-Nested PCR 1
Temp (PCR cycle):56C 1hr>94C 4min>(94C 10sec>50C 45sec>72C 45sec)*25>72C 10min
Buffer: 50mM KCl, 10mM Tris-HCl (pH 8.3), 0.1% Triton-X 100, 0.5U/microL MLV RTase, 2U/microL RNase inhibitor
Polymerase: 0.025U/microL Taq polyCycler:
Note:



Oligonucleotide information
Reverse(PN00271) primer
Oligo ID:PN00271
Oligo type: Denegeracy: 1
Target: 383-366 Tm: 54 CLength: 18
Sequence: GCCATGTACAGCAGAGAT
(3 other experimental conditions)
Note: Used with PN00270, [GI:323205].

Experimental condition used for the oligonucleotide

Common Data ID: SK0061 Publication date: 02/01
PMID: 11243658 GI: 323205 Taxonomy ID:11100
Virus Name: bovine viral diarrhea virus (isolate NADL); Bovine viral diarrhea virus genotype 2 Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.8 mM Type: RT-Nested PCR
Temp (PCR cycle):94C 10min>(94C 45sec>54C 60sec>72C 60sec)*25>72C 10min
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl
Polymerase: 0.025U/microL AmpliTaq Gold DNA polyCycler:
Note: Used with PN00270, [GI:323205].



Oligonucleotide information
Reverse(PN00424) primer
Oligo ID:pn00424
Oligo type: Denegeracy: 1
Target: 383-366 Tm: 54 CLength: 18
Sequence: GCCATGTACAGCAGAGAT
(3 other experimental conditions)
Note: Used with PN00423, [GI:9626649], in primary PCR.

Experimental condition used for the oligonucleotide

Common Data ID: BH0057 Publication date: 12/98
PMID: 9850544 GI: 902376 Taxonomy ID:54315
Virus Name: Bovine viral diarrhea virus-2, BVDV2; Bovine viral diarrhea virus (isolate NADL), BVDV-NADL Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: RT-Nested PCR
Temp (PCR cycle):94C 2min>(94C 30sec>50C 45sec>72C 60sec)*30>72C 7min
Buffer: 20mM Tris-HCl (pH 8.4), 50mM KCl
Polymerase: 0.05U/microL Taq polyCycler:
Note: Used with PN00423, [GI:9626649], in primary PCR.

Total 4 Oligos
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