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Your Name:
Your Email:
Your Institution:
PubMed ID number (eg. 11092564)
PMID:
GI for sequence (eg. 11561266)
GI:
Taxonomy ID number (eg. 8066)
Taxonomy ID:
Full name, abbreviation (eg.Bovine herpesvirus type 1.1, BHV-1.1)
Name of virus(es):
MM/YY (eg. 05/99 for an article published on May 5, 1999 Spring/00 for an article published on Spring 2000)
Date Published:
MgCl2 concentration:>
mM (eg. 2.3mM)
dNTP concentrations:
Product size:
bp (nt)(eg. 1231bp)
(eg. 94C 1min>(94C 1min>68C 1min)*40>72C 10min)(">"-next step, "*"-cycles)
PCR cycle or hybridization temp:
PCR reagents or hybridization buffer:
Type Of Assay:
N/A Asymmetric PCR cDNA synthesis DNA mobility shift assay Dot-blot Hybridization Hybridization In situ hybridization Inverse PCR Multiplex PCR Multiplex RT-PCR NASBA Nested PCR Nested RT-PCR PCR Primer extension Q-RT-PCR Real-time PCR Real-time RT-PCR Reverse Transcription RT-Asymetric PCR RT semi-nested PCR RT-Nested Multiplex PCR RT-PCR Semi-nested PCR Universal PCR Universal RT-PCR Explanations of PCR assays.
(eg. 1.5U/microL Taq poly (Promega))
Polymerase or Enzyme:
Make of PCR cycler:
RT condition:
Final volume:
Primer concentration:
Additonal note:
Name of this Oligo:
Direction: Forward Reverse
This oligo is designed for:
PCR Hybridization PCR Probe (eg. fluorescent probe, etc) Other
eg. AT(A/T)AATTCCGGT(AT/GT/AT)AA
Sequence:
Start postion and end on the reference sequence (eg. 3433)
Target Start:
Target End:
Gene Name:
Note:
