A Simple Solution for Oligonucleotide Design


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Your Name:

Your Email:

Your Institution:

PubMed ID number (eg. 11092564)

PMID:

GI for sequence (eg. 11561266)

GI:

Taxonomy ID number (eg. 8066)

Taxonomy ID:

Full name, abbreviation (eg.Bovine herpesvirus type 1.1, BHV-1.1)

Name of virus(es):

MM/YY (eg. 05/99 for an article published on May 5, 1999 Spring/00 for an article published on Spring 2000)

Date Published:

MgCl2 concentration:>

mM (eg. 2.3mM)

dNTP concentrations:

mM (eg. 2.3mM)

Product size:

bp (nt)(eg. 1231bp)

(eg. 94C 1min>(94C 1min>68C 1min)*40>72C 10min)(">"-next step, "*"-cycles)

PCR cycle or hybridization temp:

PCR reagents or hybridization buffer:

Type Of Assay:

Explanations of PCR assays.

(eg. 1.5U/microL Taq poly (Promega))

Polymerase or Enzyme:

Make of PCR cycler:

RT condition:

Final volume:

Primer concentration:

 Additonal note:


About OLIGO

Name of this Oligo:

Direction:

This oligo is designed for:

PCR
Hybridization
PCR Probe (eg. fluorescent probe, etc)
Other

eg. AT(A/T)AATTCCGGT(AT/GT/AT)AA

 Sequence:

Start postion and end on the reference sequence (eg. 3433)

 Target Start:

Target End:

 Gene Name:

 Note: