is Universal PCR?
Universal PCR means a PCR that amplifies DNA
fragments from more than one viral species, such as family- or genus-specific PCR.
|What does Nested PCR 1 or Nested PCR 2 mean?
Nested PCR in the database consists of Nested PCR 1 [First amplification],
and Nested PCR 2 [Second (nested) amplification] and so on.
|How is melting temperature (Tm) calculated?
than 19 bp oligonucleotide,
Thein SL, Lynch JR, Weatherall DJ, Wallace RB. (1986). Lancet
1 (8472): 93
length]: at salt 1000.0mM
Bolton, Et and McCarthy, B.J. (1962) PNAS 48: 139-1397