A Simple Solution for Oligonucleotide Design

 

The VirOligo database (1) is part of an effort to provide methods suitable for assessing what viruses are present in samples. Such methods should faciliate studies of the spatial and temporal distribution of viruses and their diversity at any one time and place.

To this end, we are also exploring Virus Signature Hybridization (ViSH) and Virus Signature Amplification (ViSA) (2), microarray-based methods for hybridization and PCR amplification.

Citations
1. Onodera K, Melcher U(2002) "VirOligo: a database of virus-specific oligonucleotides." Nucleic Acids Res, 30(1):203-4
2. Onodera K, d'Offay J, and Melcher U (2002) "Nylon Membrane-Immobilized PCR for Detection of Bovine Viruses." Biotechniques, 32(1):74-80

 

Project sponsors

 

S. R. Noble Foundation, Ardmore, Oklahoma
R. J. Sirny Professorship
Oklahoma Agricultural Experiment Station

 VirOLIGO Database Project  

Project Director: 

Ulrich Melcher, Ph. D.
Dept. of Biochemistry and Molecular Biology
Oklahoma State University
E-mail: ulrich.melcher@okstate.edu

Programmer:
(Spring 2003 - Present)

Yan Song, M.S.
Dept. of Biochemistry and Molecular Biology
Oklahoma State University
E-mail: yansong@biochem.okstate.edu

Curator and Programmer:
(Spring 2001 - Fall 2002)

Kenji Onodera, M.S.
Dept. of Biochemistry and Molecular Biology
Oklahoma State University
E-mail: kenji.onodera.uh@alum.riken.jp

Data Entry Supervisor:
(January 2002 - July 2002)

Brad Mons, B.S.
Dept. of Biochemistry and Molecular Biology
Oklahoma State University
E-mail: frmreldr@aol.com

Student Assistants:

 

  Fall 2005 - Spring 2006:

Josh Panzer, Biochem
Tsuyoshi Asako, Biochem

  Fall 2004 - Spring 2005:

Sarah Holle, Biochem
Susan Chapman, Biochem
Tiffani Lee, Biochem
Tsuyoshi Asako, Biochem

  Fall 2003 - Spring 2004:

Tsuyoshi Asako, (Sophomore) Biochem
Johnny Leverett, (Freshman) Biochem
Shayla Highfill, (Freshman) Biochem

  Fall 2002 - Summer 2003:

Matt Gore, Biochem
Michael Puckette, (Senior) Biochem

  Summer 2002:

Michael Puckette, (Junior) Biochem
And the VirOligo gang

  Spring 2002:

Jacob Jenkins, (Freshman) Biochem
Brian McWilliams, (Freshman) Biochem
Joshua David Powell, (Sophomore) Biochem
Michael Puckette, (Junior) Biochem
Jessica Sparks, (Freshman) Biochem

  Fall 2001:

Abudul Adeoye, (Junior) Biochem
Amber Austin (Sophomore) Biochem
Laura Clay, (Senior) Biochem
Brad Mons (Senior) Micro
Michael Puckette, (Sophomore) Biochem
Jessie Xiao, (Graduate) Computer Sci

  Summer 2001:

Amber Austin ,(Sophomore) Biochem
Brent Dale Hill ,(Graduate) Statistics
Shobana Krishnan, (Graduate) Micro
Brad Mons ,(Senior) Micro
Nelson Vining, (Sophomore) Biochem

  Spring 2001:

Amber Austin, (Freshman) Biochem
Rachael Hodson, (Freshman) Biochem
Sarah Hopping, (Senior) Micro
Hideo Ishii, (Graduate) Biochem
Shanti Kodemela, (Junior) Biochem
Brad Mons, (Senior) Micro
Monica Peters, (Freshman) Biochem
Michael Puckette, (Sophomore) Biochem
Shelly Wilson, (Freshman) Biochem
Matthew Thorn, (Freshman) Biochem
Clint Wichert, (Junior) Biochem
Valerie Wittenmyer, (Freshman) Biochem

Fall 2000:

Abudul Adeoye, (Junior) Biochem